Kisspeptin Peptide: What It Is, Dosage and Testosterone

Kisspeptin sits at the very top of the testosterone chain. It is the signal that tells the brain to release GnRH, which tells the pituitary to release LH and FSH, which tell the testes to make testosterone. It works on your own system rather than replacing testosterone, which is the main difference from TRT.

• What it is: Peptides from the KISS1 gene; kisspeptin-10 (10 amino acids) is the one sold
• FDA status: Never approved; kisspeptin-10 is on the FDA’s compounding safety-risk list
• Sport: Banned at all times in men by WADA (S2.2.1)
• Human studies: Small trials: LH and testosterone rise, IVF egg maturation
• What people take: 200-330 mcg a shot (Regimen users), 200 mcg most common
• Common vial: 10 mg, mixed with 2 or 3 mL
• Half-life: About 4 minutes (kisspeptin-10), 28 minutes (kisspeptin-54)

What Is Kisspeptin?

Kisspeptins are a family of peptides made from one gene, KISS1. The body cuts the same starting protein into kisspeptin-54, -14, -13 and -10, which share the same last 10 amino acids. Kisspeptin-54 is the main form in human blood. Kisspeptin-10 is the shortest piece that still works fully, and it is the form sold as a peptide.

It acts on a receptor in the brain (GPR54, also called KISS1R) and releases the brain’s own store of GnRH. GnRH then drives LH and FSH from the pituitary, and LH drives testosterone. Kisspeptin is needed for puberty, and it was first found as a gene that held back the spread of cancer cells, which is why the 54-amino-acid form is also called metastin.

Does Kisspeptin Raise Testosterone?

In men, short-term studies say yes:

• Kisspeptin-54 by IV (2005): in 6 healthy men, LH more than doubled and testosterone rose about 15%.
• Kisspeptin-10 by IV (2011): a single dose raised LH, and a 22.5-hour infusion raised testosterone about 45% in 4 men.
• Men with type 2 diabetes and low testosterone (2013): an infusion raised testosterone by about a third.
• Infusions under the skin (2026): in 7 men, 8 hours on and 16 hours off kept LH, FSH and testosterone up for 12 days.

How often you dose matters. In women with amenorrhea (no periods) given kisspeptin-54 twice a day, the LH response had almost gone by day 14 (from 24.0 to 2.5 IU/L). Twice-weekly dosing only partly dulled it. And in men on a continuous 5-day infusion, LH and FSH fell back to placebo levels, though testosterone stayed raised. More often is not better.

Kisspeptin Dosage (Kisspeptin-10): How Much and How Often

There is no approved kisspeptin-10 dosage. The human studies dosed by body weight, mostly by IV:

• Kisspeptin-10 by IV: the LH response peaked at 1 mcg per kg, and 3 mcg per kg gave a smaller response.

What people take: most Regimen users on kisspeptin take 200 to 330 mcg a shot, and 200 mcg is the most common dose. 39% take it every day and 30% three days a week; most of the rest take it every other day or twice a week.

How to Reconstitute Kisspeptin (Units to Draw)

73% of Regimen users on kisspeptin use a 10 mg vial, and most mix it with 3 or 2 mL of bacteriostatic water. Kisspeptin dosage chart, in units on a 100-unit (1 mL) insulin syringe:

With a 5 mg vial and 2 mL, 200 mcg is 8 units. At 2 to 6 units, a small misread is a big share of the dose. More water means more units for the same dose, which makes small doses easier to measure. For any other mix, use the peptide calculator.

Kisspeptin Side Effects

In the human studies, kisspeptin was well tolerated: the recent trials in men and women with low sexual desire reported no side effects. In an IVF trial in women at high risk of ovarian overstimulation, no one developed moderate or severe overstimulation.

These were small, short studies. The FDA put kisspeptin-10 on its compounding safety-risk list (Category 2) in 2023, saying it may cause immune reactions by some routes, can be hard to make without impurities, and has little safety data for the proposed routes. Long-term effects are not known.

Kisspeptin vs TRT, hCG and PT-141

• TRT supplies testosterone from outside. See the testosterone cypionate dosage guide.
• hCG acts like LH directly on the testes. See hCG for men on TRT.
• PT-141 (bremelanotide) works on melanocortin receptors in the brain, not on hormones. As Vyleesi, it is FDA-approved for low sexual desire in premenopausal women.
• Kisspeptin works further up, in the brain, so the whole chain from GnRH down runs on your own hormones. Unlike TRT and hCG, it isn’t an approved drug.

Among Regimen users on kisspeptin, 24% also track testosterone, 23% PT-141 and 16% hCG.

Kisspeptin Benefits for Fertility and Libido

In IVF trials, a single shot of kisspeptin-54 under the skin (1.6 to 12.8 nmol per kg) triggered egg maturation: in 60 women at high risk of overstimulation, 95% had mature eggs. A second dose 10 hours later raised the share of women with a good egg yield from 45% to 71%. In women early in their cycle, kisspeptin-10 did not raise LH even at high doses. Kisspeptin also changed how the brain processes sexual cues in small studies of men and women with low sexual desire.

How Long to Run Kisspeptin

34% of Regimen users on kisspeptin run it in cycles, most often 4 or 8 weeks on with 2 to 4 weeks off.

Where to Inject Kisspeptin

Kisspeptin goes under the skin. Most Regimen users on kisspeptin inject in the belly or the thigh. The peptide injection sites guide shows each spot.

Is Kisspeptin Legal?

Kisspeptin has never been approved by the FDA, and kisspeptin-10 is still on the FDA’s compounding safety-risk list (Category 2), so US compounding pharmacies are not supposed to make it. The 2026 WADA Prohibited List names kisspeptin and its analogues among testosterone-stimulating peptides banned at all times in men (S2.2.1).

Track Kisspeptin With Regimen

Kisspeptin is about what happens downstream, so the numbers that matter are on your bloodwork. Regimen, a peptide tracker, puts testosterone, estradiol (E2) and SHBG, scanned or typed in, on the same timeline as your doses; the TRT blood work guide covers what to test.

For more compounds, see the list of peptides. This article is for education and tracking. It is not medical advice.

Sources

• Structure, forms, puberty and metastin: Clarke, Dhillo and Jayasena, 2015; d’Anglemont de Tassigny et al., 2017; Chakraborty et al., 2025.
• Men, LH and testosterone: Dhillo et al., 2005; George et al., 2011; George et al., 2013; Yeung et al., 2026.
• Half-life and women: Jayasena et al., 2011.
• Desensitization: Jayasena et al., 2009; Jayasena et al., 2010.
• IVF: Jayasena et al., 2014; Abbara et al., 2015; Abbara et al., 2017.
• PT-141: Vyleesi prescribing information, DailyMed. Sexual desire studies: Mills et al., JAMA Network Open, 2023; Thurston et al., JAMA Network Open, 2022.
• Compounding: FDA, bulk drug substances that may present significant safety risks. Sport: WADA 2026 Prohibited List.
• Regimen app data: the current kisspeptin protocol of each Regimen user (dose, schedule, vial, water, cycles), other compounds on the same account, and injection sites logged in the 180 days to October 27, 2026.

All sources checked October 27, 2026.