Intranasal Peptide Calculator

Enter your peptide concentration, spray volume, and target dose, get the number of sprays per administration and bottle longevity.

Intranasal peptides

Nasal spray dosing

Sprays per dose, concentration, and how long a bottle lasts.

Quick Presets

Peptide Amount

Liquid Volume (mL)

Spray Volume per Actuation (mL)

Most nasal sprays deliver 0.1mL per spray

Target Dose per Administration

How many times per day?(for bottle longevity)

This calculator is for informational purposes only, so always double-check your result with a healthcare provider. We assume no liability for dosing errors.

The tool

How it works, what it tells you, what it doesn't.

The intranasal calculator tells you exactly how many sprays to use per dose for nasal peptide preparations like BPC-157, Selank, Semax, and NAD+. Each actuation of a nasal spray pump delivers a fixed volume (typically 0.1 mL). Enter your preparation details and the calculator shows dose per spray, sprays needed for your target, total sprays per bottle, and estimated days of supply at your dosing frequency.

How to use it

How to Use This Calculator

01

Enter peptide amount

The total amount of peptide in your solution (mg or mcg)
02

Enter liquid volume

Total volume of solution in your spray bottle (mL)
03

Set spray volume

Volume per spray actuation (typically 0.1mL)
04

Enter target dose

How much peptide you want per administration

How Peptide Nasal Sprays Work

Intranasal delivery sends peptides directly through the nasal mucosa - the thin, highly vascularized tissue lining your nasal passages. Because this tissue is rich in blood vessels and has a large surface area, peptides can absorb rapidly into systemic circulation, bypassing the digestive system entirely.

Not every peptide works well as a nasal spray. The best candidates share a few characteristics:

Molecular weight under ~6,000 Daltons. Smaller molecules pass through the nasal mucosa more efficiently.
Some degree of lipophilicity. Peptides that are moderately fat-soluble cross the nasal membrane more readily.
Stability in solution. The peptide needs to remain active in a water-based solution at nasal pH for a reasonable shelf life.

Absorption rates generally fall between 5-20% bioavailability nasally, compared to near-zero orally and close to 100% via injection. The trade-off between convenience and bioavailability is why nasal doses are typically higher than injectable doses for the same peptide.

Common Peptide Nasal Spray Doses

The table below covers typical nasal dosing for commonly used peptides. These are general reference ranges - your specific dose may differ based on your protocol.

Peptide	Typical Concentration	mcg per Spray (0.1 mL)	Sprays per Dose	Doses per Day	Common Daily Total
BPC-157	2,000 mcg/mL	200 mcg	1-2	2	400-800 mcg
Semax (0.1%)	1,000 mcg/mL	100 mcg	1-2	2-3	200-600 mcg
Selank	1,500 mcg/mL	150 mcg	1-2	2-3	300-900 mcg
DSIP	1,000 mcg/mL	100 mcg	1	1 (before bed)	100 mcg
PT-141	5,000 mcg/mL	500 mcg	2-4	As needed	1,000-2,000 mcg
Oxytocin	100 IU/mL	10 IU	1-2	1-2	10-40 IU
NAD+	100 mg/mL	10 mg	1-2	1-2	10-40 mg

Concentrations listed above are chosen so that each 0.1 mL spray delivers a practical single-spray dose. When reconstituting your own peptide, you control the concentration by adjusting how much diluent you add.

How to Prepare a Peptide Nasal Spray

Reconstitute the peptide. Add your chosen diluent (bacteriostatic water or sterile 0.9% sodium chloride) to the peptide vial. Aim the stream down the inside wall and let it dissolve gently - do not shake.
Calculate your concentration. Divide the total peptide content (in mcg) by the total diluent volume (in mL). For example, 5,000 mcg of BPC-157 reconstituted in 2.5 mL gives you 2,000 mcg/mL.
Transfer to the nasal spray bottle. Use a syringe to draw the reconstituted solution and transfer it into a clean nasal spray bottle. Standard bottles deliver approximately 0.1 mL per actuation.
Prime the sprayer. Before first use, pump several times until a fine mist appears. You will typically waste 3-5 sprays during priming - account for this when calculating total doses.
Calculate total doses per bottle. Divide the total volume in your bottle by 0.1 mL to get the number of sprays. Then divide by your sprays-per-dose to find how many doses the bottle contains.

For a deeper walkthrough of reconstitution math, see our Peptide Reconstitution Calculator Guide.

Logging each spray and rotating nostrils gets tedious fast. Compare the best peptide tracker apps if you want intranasal protocols tracked alongside injectables.

Storage and Shelf Life

Reconstituted peptides are far less stable than lyophilized (freeze-dried) powder. Proper storage directly affects how long your nasal spray remains effective.

Refrigerate at all times. Store your nasal spray bottle at 2-8°C (standard refrigerator temperature). Do not freeze. Remove it only briefly for dosing, then return it immediately.
Protect from light. Many peptides are photosensitive. Use an amber or opaque nasal spray bottle if available.
Expect 28-30 days of stability. Once reconstituted, most peptides remain stable for approximately four weeks when refrigerated. When in doubt, prepare smaller batches more frequently.
Signs of degradation: Cloudiness, particulate matter, unusual color change, or a different smell all suggest the solution has degraded. Discard and prepare a fresh batch.

Tips for Better Nasal Absorption

How you administer a nasal spray affects how much peptide actually reaches your bloodstream. These practical tips help maximize absorption:

Clear your nasal passages first

Blow your nose gently or use a saline rinse 5-10 minutes before dosing. Mucus blocks absorption.

Alternate nostrils

If your dose requires two sprays, put one in each nostril. This doubles the absorptive surface area.

Tilt your head slightly forward

A gentle forward tilt helps direct the spray onto the nasal mucosa rather than down your throat.

Do not sniff hard after spraying

A sharp sniff pulls the solution past the mucosa into your throat. Breathe in gently through your nose.

Wait 30-60 seconds between sprays

Give each spray time to absorb before administering the next to prevent pooling and dripping.

Avoid blowing your nose for 15 minutes

Give the peptide time to fully absorb before clearing your nasal passages.

Track Your Nasal Peptide Protocol

Nasal peptide protocols often involve multiple compounds, varying doses, and subtle effects that are hard to evaluate without data. Tracking makes the difference between guessing and knowing what works.

Peptide Reconstitution Calculator

Calculate reconstitution volumes for any peptide

Semax Nasal Spray Dosing Guide

Detailed Semax protocol reference

Peptide Reconstitution Guide

Step-by-step reconstitution walkthrough

Get the app

Track Your Peptide Protocol

Regimen logs every dose, tracks reconstitution dates, and reminds you when to administer. Free for one compound.

Log intranasal and injectable doses
Smart dosing reminders
Track effects and progress over time
Private, secure health data

Free to download. Free for your first compound.

Regimen app Today view with doses and estimated levels

Questions

Frequently Asked Questions

What is an intranasal peptide?

An intranasal peptide is a peptide administered via nasal spray rather than injection. Common examples include BPC-157, Semax, Selank, and PT-141. The nasal mucosa allows direct absorption into the bloodstream and, for some peptides, more efficient crossing of the blood-brain barrier.

How do I calculate my intranasal peptide dose?

Enter your vial size (mg), the volume of bacteriostatic water used to reconstitute it (mL), and your target dose (mcg) into the calculator. It will calculate the exact volume per spray. Most nasal spray pumps deliver 0.1mL per actuation - the calculator tells you how many pumps equals your dose.

What peptides can be taken intranasally?

Common intranasal peptides include BPC-157 (healing and gut health), Semax (cognitive enhancement), Selank (anxiety and cognition), PT-141 (sexual function), and Epitalon (longevity). Not all peptides are suitable for intranasal delivery - some are too large to cross the nasal mucosa effectively.

How is intranasal dosing different from subcutaneous injection?

Intranasal delivery absorbs through the nasal mucosa and can cross the blood-brain barrier more efficiently for certain peptides. Subcutaneous injection delivers the peptide into fat tissue for systemic absorption. Intranasal typically requires a higher dose than subcutaneous for equivalent effect, since mucosal absorption is less complete than injection.

What concentration should I use when reconstituting peptides for intranasal use?

For intranasal use, most practitioners reconstitute at 1–2mg/mL. A 5mg vial reconstituted with 5mL of bacteriostatic water gives 1mg/mL. With a standard nasal pump delivering 0.1mL per spray, each spray delivers 100mcg. Adjust your reconstitution volume to hit your target dose per spray.

How do intranasal peptides work?

Intranasal peptides are absorbed through the nasal mucosa directly into the bloodstream, bypassing the digestive system. This route provides faster absorption and higher bioavailability for certain peptides compared to oral administration.

What is a typical spray volume?

Most nasal spray devices deliver 0.1mL (100 microliters) per actuation. Some devices deliver 0.05mL or 0.13mL. Check your spray bottle specifications for the exact volume.

Can BPC-157 be taken intranasally?

Yes, BPC-157 has been studied via intranasal administration. Typical doses range from 100-500mcg per administration, usually taken 1-2 times daily. The intranasal route may be preferred for systemic and neurological applications.

How long does a reconstituted nasal spray last?

Reconstituted nasal sprays typically last 2-4 weeks when stored in the refrigerator. Use preservative-free saline as the mixing medium. Always discard the solution if it becomes cloudy, changes color, or develops particulate matter.

What's the difference between bacteriostatic water and saline for nasal sprays?

Bacteriostatic water contains 0.9% benzyl alcohol as a preservative, which can irritate nasal passages. Preservative-free saline (0.9% sodium chloride) is the preferred diluent for intranasal sprays because it matches your body's natural salinity and is gentler on the nasal mucosa.

How long does a nasal spray bottle last?

This depends on your dose frequency and the total liquid volume. Use the 'doses per day' field in the calculator to see your estimated bottle longevity. For example, a 10mL bottle with 0.1mL sprays at 2 sprays twice daily lasts about 25 days.

What peptides work best intranasally?

BPC-157, Selank, Semax, and NAD+ are the most commonly administered intranasal peptides. Each has different typical dosing ranges, which you can see in the reference table on this calculator.