TB-500 Reconstitution Calculator — How to Mix & Dose TB-500

TB-500 is basically your body's repair crew on overdrive. It's built from a protein you already make naturally (Thymosin Beta-4), but the synthetic version is concentrated for one job: getting repair cells to injuries faster and calming inflammation while they work.

What Is TB-500?

TB-500 is basically your body's repair crew on overdrive. Your body already makes a protein called Thymosin Beta-4 (TB4) that helps fix damaged tissue. TB-500 is a synthetic fragment of that protein, specifically the 17-amino-acid active piece that does most of the healing work.

Here's what it actually does in plain terms: it helps your repair cells get to the injury faster and grow new blood vessels once they arrive. The way it pulls this off is by boosting production of actin, which is the protein your cells need to move around and rebuild. More actin means cells can migrate to the damage site quicker. And the new blood vessel growth (called angiogenesis) means more blood flow to the injured area, which means more oxygen and nutrients where you need them. On top of that, it dials down inflammation through its own set of pathways.

TB-500 comes as a freeze-dried powder in vials (most commonly 2mg, 5mg, and 10mg). One thing that makes it different from BPC-157: instead of running a flat dose from day one, TB-500 uses a two-phase protocol. You start with a higher-dose loading phase for 4-6 weeks, then drop to a lower maintenance phase. That loading/maintenance structure is one of the defining features of TB-500 dosing.

What You Need to Reconstitute

Before mixing, gather everything:

Step-by-Step Reconstitution Process

1. Swab the tops of both vials (TB-500 and BAC water) with alcohol. Let them dry completely. Takes about 15-30 seconds.
2. Draw your chosen amount of BAC water into the syringe. Common amounts: 1mL for the 2mg vial, 2mL for the 5mg and 10mg vials.
3. Insert the needle into the TB-500 vial at an angle. Aim the stream of water at the inside glass wall, not directly onto the powder cake. Peptides are fragile molecules and direct force can damage the peptide structure.
4. Push the plunger slowly. Let the water run down the side of the vial. Do NOT shake. Gently swirl the vial or roll it between your palms until the powder is fully dissolved.
5. Check the solution. Once completely clear with no visible particles or cloudiness, you're good to go. Your TB-500 is reconstituted and ready to dose.

Dose Calculation: The Math Explained

TB-500 is dosed in milligrams (mg), but your syringe is marked in units. Here's how to connect them.

Worked Example

• You have a 5mg TB-500 vial
• You add 2mL of BAC water
• Concentration = 5mg ÷ 2mL = 2.5 mg/mL
• You want a 2.5mg dose (common loading dose)
• Units to draw = (2.5 ÷ 2.5) × 100 = 100 units on your insulin syringe (a full 1mL syringe)

That 5mg vial at 2.5mg per injection gives you 2 doses. That's one week of loading if you inject 2.5mg twice per week, or two weeks if you inject 2.5mg once per week.

Or skip the math entirely. Use our Peptide Reconstitution Calculator to get the exact number of units for any vial size, BAC water volume, and target dose. For help reading your syringe markings accurately, see our insulin syringe reading guide.

TB-500 Dose Charts

5mg Vial + 2mL BAC Water (2.5 mg/mL)

10mg Vial + 2mL BAC Water (5.0 mg/mL)

Loading vs Maintenance Protocol

This is the thing that makes TB-500 different from most recovery peptides. Instead of a flat daily dose like BPC-157, TB-500 uses a two-phase approach: hit it hard during loading to saturate your tissues, then drop to a lower maintenance dose to keep things going.

How the loading phase works: TB-500 has a short half-life in your blood (roughly 3-4 hours), but its effects on cell repair and migration last longer than the peptide itself stays in circulation. The loading phase front-loads a higher amount so enough peptide actually reaches your tissues and kicks off the repair process. Think of it like priming a pump.

How maintenance works: After 4-6 weeks of loading, you drop to a lower dose once every 1-2 weeks. You're not starting the repair process anymore, you're just sustaining the environment you built during loading.

When to extend loading: For more severe or chronic injuries (long-standing tendon issues, post-surgical recovery, significant tissue damage), some protocols run loading for 6-8 weeks before switching to maintenance.

TB-500 + BPC-157 Stack Guide

TB-500 and BPC-157 are the two most commonly stacked recovery peptides. The community calls it "the recovery stack," and for good reason: they work through completely different mechanisms, so running them together hits recovery from multiple angles.

BPC-157 focuses on growing new blood vessels, speeding up tendon and ligament repair, and protecting the GI tract. TB-500 focuses on moving repair cells to the injury, reducing inflammation through different pathways, and supporting broader tissue remodeling. Together, BPC-157 builds the vascular infrastructure for healing while TB-500 gets repair cells to the site and manages inflammation.

Both can be injected on the same day. BPC-157 runs daily while TB-500 runs twice per week during loading. On days you inject both, you can do them at the same time, but draw them into separate syringes from their individual vials. Don't mix reconstituted peptides from two vials into one syringe for storage.

If you're using a pre-blended BPC-157/TB-500 product from a compounding source, use our Peptide Blend Calculator to figure out per-peptide dosing from a single vial.

For the full breakdown of how to structure the BPC-157 + TB-500 stack, see our BPC-157 + TB-500: The Ultimate Recovery Stack Guide. For BPC-157 specifically, see our BPC-157 Reconstitution Calculator Guide.

Track your TB-500 protocol, doses, and recovery progress

• Log loading and maintenance phases side by side
• Smart reminders for twice-weekly loading doses
• Track recovery timeline and progress photos

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Injection Sites for TB-500

TB-500 goes subcutaneous (SubQ). Here are the most common injection sites:

Abdomen (standard SubQ): Lower belly, at least 2 inches from the navel. This is the go-to for TB-500, same as BPC-157 and most other SubQ peptides. Easy access, consistent absorption, and the least painful spot for most people.

Near the injury site: A lot of users inject TB-500 subcutaneously as close to the injury as they can. The logic is that local injection delivers higher concentrations right where you need them. This is popular but debated: TB-500 is systemically active regardless of injection site, and there's no controlled human data showing local injection actually works better than a standard belly shot.

Rotation: Wherever you inject, rotate your sites. Don't keep hitting the same exact spot. For a visual map of injection sites, rotation patterns, and technique tips, see our Peptide Injection Sites Guide.

Storage After Reconstitution

Never freeze reconstituted TB-500. Keep the vial upright in the fridge, away from direct light. If the solution turns cloudy, develops floating particles, or changes color, toss it and mix a fresh vial. For a full breakdown of peptide storage best practices, see our Peptide Storage Guide.

Side Effects

TB-500 has a pretty mild side-effect profile. Serious adverse effects are uncommon based on community reports. That said, formal human safety data is limited, so pay attention to how your body responds.

Most side effects show up during the first week of loading and fade as your body adjusts. If they stick around past week one or get worse, lower your dose and talk to a healthcare provider. Track your side effects alongside your doses in the Regimen app to spot patterns and dial in your protocol.

Common Reconstitution Mistakes

Frequently Asked Questions