BPC-157 Reconstitution Calculator — How to Mix & Dose BPC-157

BPC-157 is one of the most popular peptides for injury recovery, and reconstituting it is straightforward. The catch is that dosing happens in micrograms, not milligrams, which means one wrong decimal point is a 1,000x error. This guide walks you through the reconstitution process, the math, and the most common dosing protocols.

What Is BPC-157?

BPC-157 (Body Protection Compound-157) is a synthetic peptide derived from a protective protein found in human gastric juice. It consists of 15 amino acids and has been studied extensively in animal models for its effects on tissue repair, wound healing, tendon and ligament recovery, gut healing, and inflammation reduction.

It's one of the most widely used peptides in the recovery and performance space. Unlike GLP-1 compounds that require slow titration, BPC-157 is run at a flat dose from day one, typically 250-500mcg injected once or twice daily, with most protocols running 4-8 weeks.

BPC-157 comes as a lyophilized (freeze-dried) powder in vials ranging from 5mg to 15mg. You reconstitute it with bacteriostatic water before injecting subcutaneously or intramuscularly near the site of injury.

What You Need to Reconstitute

Before mixing, gather everything:

Step-by-Step Reconstitution Process

1. Clean the tops of both vials (BPC-157 and BAC water) with alcohol swabs. Let dry for 10 seconds.
2. Draw your chosen amount of BAC water into the syringe. Common amounts: 1mL or 2mL for 5mg vials, 2mL for 10mg vials, 3mL for 15mg vials.
3. Insert the needle into the BPC-157 vial at a slight angle, aiming the stream at the glass wall. Never aim directly onto the powder cake. Peptides are fragile molecules and direct force can degrade them.
4. Depress the plunger slowly, letting the water run down the side of the vial. Do NOT shake. Gently swirl the vial or roll it between your palms until the powder is fully dissolved. BPC-157 typically dissolves within 30-60 seconds.
5. Check the solution. Once completely clear with no visible particles or cloudiness, your BPC-157 is reconstituted and ready to dose.

Dose Calculation: The Math Explained

BPC-157 is dosed in micrograms (mcg), but your vial is labeled in milligrams (mg) and your syringe is marked in units. Here's how to connect them.

Worked Example

• You have a 5mg BPC-157 vial = 5,000mcg total
• You add 2mL of BAC water
• Concentration = 5,000mcg ÷ 2mL = 2,500mcg/mL
• You want a 500mcg dose
• Units to draw = (500 ÷ 2,500) × 100 = 20 units on your insulin syringe

That gives you 10 doses of 500mcg from one 5mg vial (with 2mL BAC water).

Or skip the math entirely. Use our Peptide Reconstitution Calculator to get the exact number of units for any vial size, BAC water volume, and target dose combination.

Common Vial Sizes and Dose Charts

5mg Vial + 2mL BAC Water (2,500 mcg/mL)

10mg Vial + 2mL BAC Water (5,000 mcg/mL)

15mg Vial + 3mL BAC Water (5,000 mcg/mL)

Using the BPC-157 Reconstitution Calculator

Instead of doing the math manually, use our free Peptide Reconstitution Calculator:

1. Select "BPC-157" as the compound
2. Enter your vial size (5mg, 10mg, or 15mg)
3. Enter the amount of BAC water you added (in mL)
4. Enter your target dose in mcg (e.g., 250 or 500)
5. The calculator shows exactly how many units to draw on your insulin syringe
6. It also shows total doses per vial so you can plan your cycle supply

The calculator supports all common peptides, including TB-500, GHK-Cu, and more. You can save your calculations and track your doses over time in the Regimen app.

Track your BPC-157 doses, cycle length, and recovery progress

• Log injection sites near specific injuries
• Set twice-daily reminders for split-dose protocols
• Track pain levels and recovery progress over time

Get Regimen Free

BPC-157 Dosing Protocols

Unlike GLP-1 compounds, BPC-157 does not require titration. You start at your target dose from day one. The dose you choose depends on what you're targeting.

Targeted Injury Recovery (Tendon, Ligament, Muscle)

This is the most common BPC-157 protocol. The twice-daily dosing compensates for BPC-157's short half-life (~4 hours) and maintains more consistent tissue-level exposure throughout the day.

General Healing / Gut Health

For gut-related applications (leaky gut, IBS symptom management, NSAID-related GI damage), abdominal subcutaneous injection is preferred. Some users report benefit from oral BPC-157 for gut-specific applications, though injectable remains the standard research route.

Maintenance / Preventive

Lower-dose maintenance protocols are used between intensive cycles or for general tissue support. This is not a well-established protocol in the literature. It comes from community practice.

Stacking BPC-157 with TB-500

BPC-157 is frequently stacked with TB-500 (Thymosin Beta-4) for synergistic recovery effects. The two peptides work through different mechanisms. BPC-157 primarily promotes angiogenesis and tendon/ligament repair, while TB-500 supports broader tissue repair and reduces inflammation through different pathways.

TB-500 has a much longer half-life than BPC-157, so it only needs to be injected 1-2 times per week. They can be injected at the same time but should be drawn into separate syringes unless you're using a pre-blended product.

For the full breakdown of how these two peptides complement each other, dosing timelines, and cycle structures, see our BPC-157 + TB-500 Recovery Stack Guide.

If you're using a pre-mixed BPC-157/TB-500 blend, use our Peptide Blend Calculator to calculate per-peptide dosing from a single vial.

Storage After Reconstitution

Never freeze reconstituted BPC-157. Keep the vial upright in the refrigerator, away from direct light. If the solution develops cloudiness, particles, or discoloration after storage, discard it.

For a full breakdown of peptide storage best practices, temperature ranges, and travel tips, see our Peptide Storage Guide.

Cycling: How Long to Run BPC-157

BPC-157 is typically run in defined cycles rather than continuously. Standard practice:

Most users run one or two 4-8 week cycles for a specific injury and then stop. There's no established long-term safety data for continuous BPC-157 use in humans, so cycling off is the consensus approach in the community.

For more on peptide cycling structures, on/off ratios, and how to plan multi-peptide cycles, see our Peptide Cycling Guide.

Side Effects

BPC-157 has a relatively mild side-effect profile compared to many other peptides. Reported side effects are uncommon but include:

Track your side effects alongside your doses in the Regimen app to identify patterns and adjust your protocol accordingly.

Common BPC-157 Reconstitution Mistakes to Avoid

Frequently Asked Questions