The Bottom Line
TB-500 is a synthetic fragment of Thymosin Beta-4, a naturally occurring peptide found in nearly all human and animal tissues. It is widely used for tissue repair and recovery. In April 2026 it was among the 12 peptides removed from the FDA’s Category 2 list; it is still an unapproved drug, and an FDA advisory committee voted in July 2026 to recommend it for compounding, but it cannot legally be compounded until the FDA completes rulemaking.
Most people run TB-500 in two phases: a higher loading dose for the first 4-6 weeks, then a lower maintenance dose.
TB-500 Dosage Chart
TB-500 dosage per week is 4-5 mg during the loading phase, split into two injections of 2-2.5 mg, then 2-2.5 mg a week for maintenance. TB-500 is dosed in milligrams (mg), not micrograms. Vials are typically sold as 5mg lyophilized powder.
TB-500 (TB500) dosing chart
| Protocol Phase | Dose | Frequency |
|---|---|---|
| Loading phase | 4-5 mg a week (2-2.5 mg per shot) | Twice weekly for 4-6 weeks |
| Maintenance | 2-2.5 mg a week | Once weekly, or split into two shots |
The loading phase is meant to build up tissue saturation; after it, people drop to maintenance. Most run TB-500 for 8-16 weeks in total.
How to Reconstitute TB-500
TB-500 is a lyophilized powder that requires reconstitution with bacteriostatic water (BAC water).
Standard reconstitution for a 5mg vial:
| BAC Water Added | Concentration | Volume per 2.5mg dose |
|---|---|---|
| 1mL | 5mg/mL | 0.5mL (50 units on insulin syringe) |
| 2mL | 2.5mg/mL | 1.0mL |
| 2.5mL | 2mg/mL | 1.25mL |
Recommended: Add 1-2mL of BAC water to a 5mg vial. This gives a concentration that keeps injection volumes manageable.
Steps:
- Wipe vial top with alcohol swab
- Draw BAC water into syringe
- Inject water slowly down the inside wall of the vial; do not aim directly at the powder
- Swirl gently until dissolved
- Store refrigerated (2-8 degrees C), use within 21 days
Use the peptide reconstitution calculator to calculate the exact volume per injection for any dose.
Injection Protocol
TB-500 is injected subcutaneously or intramuscularly:
- SubQ: 27-31g insulin syringe, abdomen or thigh. Most common approach.
- IM: Some practitioners prefer IM for TB-500 due to the larger volume involved with higher doses.
- Site: Unlike BPC-157, TB-500 is generally injected systemically (not near the injury site): it circulates and acts on tissues throughout the body.
- Frequency: Twice weekly during loading; once or twice weekly for maintenance.
TB-500 vs BPC-157: How They Differ
| TB-500 | BPC-157 | |
|---|---|---|
| Mechanism | Actin-binding, cell migration | Growth factor upregulation, angiogenesis |
| Dose unit | mg | mcg |
| Dosing frequency | Once or twice weekly | Daily or twice daily |
| Injection site | Systemic (anywhere) | Near injury site or systemic |
| Primary use case | Systemic tissue repair, inflammation | Tendon, gut, localized healing |
| Stacking | Commonly stacked with BPC-157 | Commonly stacked with TB-500 |
Stacking TB-500 with BPC-157
The “healing stack” of TB-500 + BPC-157 is one of the most common peptide protocols used for injury recovery. The rationale: they work through complementary mechanisms, with TB-500 providing systemic anti-inflammatory and cell migration effects while BPC-157 promotes localized angiogenesis and growth factor upregulation.
Common stacking protocol:
- BPC-157: 250-500 mcg a day SubQ, as one shot or split in two (near injury site if applicable)
- TB-500: 2-2.5 mg SubQ twice weekly for the 4-6 week loading phase, then 2-2.5 mg a week, systemic site
Most people run both for 8-16 weeks. Some practitioners continue BPC-157 on a maintenance dose while tapering off TB-500. For the same pair run as the Wolverine stack, see the Wolverine stack dosage guide.
BPC-157 + TB-500 Blend Dosage Per Day
A pre-mixed BPC-157 + TB-500 blend holds both peptides in one vial at a locked ratio, so the blend dosage per day is set by one number: how much BPC-157 you want per shot. The simplest way to dose a blend is by its BPC-157 side: 250-500 mcg a day, as one shot or split in two, and the TB-500 comes along with it. In a vial with equal amounts of each, that is 250-500 mcg of TB-500 a day, about 1.75-3.5 mg a week: close to a maintenance dose, below a loading dose.
You can’t raise the TB-500 on its own in a blend. If you want a TB-500 loading phase, you need separate vials. To see the units to draw and exactly what each shot of your blend delivers, use the BPC-157 TB-500 blend dosage calculator.
Tracking Your TB-500 Protocol
TB-500 is typically used for a specific purpose: an injury, chronic inflammation, or post-surgical recovery. Tracking your protocol and response gives you actionable data instead of guesswork.
What to track:
- Dose and injection schedule
- Subjective pain or mobility score (1-10)
- Sleep quality and resting heart rate (from Apple Health or Google Health Connect)
- Weekly progress notes
Tracking TB-500 with an App
What’s the best app for tracking TB-500?
Regimen is a purpose-built tracker for TB-500 that logs each injection with dose, site rotation, and time. It is free for one compound, no time limit; Premium if you track more than one. The free tier includes half-life tracking, injection site rotation, dose reminders, and your weight, body composition and sleep from Apple Health or Health Connect on the same timeline as your doses, so you can see how TB-500 is affecting them over time.
Can I track TB-500 in Apple Health?
Apple Health doesn’t have native TB-500 tracking. Regimen logs your TB-500 doses in the app and pulls weight, body fat and sleep in from Apple Health, so you can see them next to your doses. It reads from Apple Health; it doesn’t write your doses back.
How do I track TB-500 side effects?
A tracker app with structured markers (energy, sleep, recovery, soreness) is more reliable than a notes app. Regimen tracks side effects and 50+ daily markers on the same timeline as your dose log, so patterns show up as the weeks add up.
Frequently Asked Questions
What is the dosage for TB-500?
The usual dosage of TB-500 has two phases: a loading phase of 4-5 mg a week (2-2.5 mg twice weekly) for the first 4-6 weeks, then a maintenance dose of 2-2.5 mg a week. Most people run it for 8-16 weeks in total. TB-500 is dosed in mg, not mcg, and is usually injected under the skin at a site away from the injury, because it acts throughout the body.
What is the BPC-157 TB-500 blend dosage per day?
In a pre-mixed BPC-157 + TB-500 blend the ratio is locked, so the dosage per day is set by the BPC-157 side: 250-500 mcg a day, as one shot or split in two. In a vial with equal amounts of each, that is about 1.75-3.5 mg of TB-500 a week. The BPC-157 TB-500 blend dosage calculator shows the units to draw and what each shot delivers.
TB-500 is commonly stacked with BPC-157 for enhanced recovery. See the April 2026 FDA ruling for where compounding stands after the July 2026 vote.
Regimen is a tracking tool, not a medical service. We do not provide medical advice. Always consult your healthcare professional before starting, changing, or stopping any medication protocol.
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